SUBJECT

Prokaryotic Gene Regulation

lecture

master

Faculty of Science

Biology MSc

2

Semester 3

Autumn semester

1. Molecular mechanism of transcription I. Organisation of prokaryotic genes. Structure and function of RNA polymerase.

2. Molecular mechanism of transcription II. Transcription initiation, elongation, and termination. Elements of prokaryotic promoters.

3. Strategies for transcriptional regulation I. Positive and negative control. Transcription factors.

4. Strategies for transcriptional regulation II. Alternative sigma factors. Turning genes on and off by recombination.

5. Molecular mechanisms in the regulation of transcription initiation I. Repression of gene transcription. Steric hindrance, caging, contact inhibition.

6. Molecular mechanisms in the regulation of transcription initiation II. Activation of transcription initiation.

7. Molecular mechanisms in the regulation of transcription initiation III. Multipartite operators. Assembly of higher-order DNA-protein comlexes. DNA looping.

8. Regulation of transcription elongation and termination. Natural and mutational polarity. Transcription attenuation.

9. Molecular mechanism of translation. Initiation, elongation, and termination of translation. Structure and function of Ribosome.

10. Strategies and mechanisms in regulation of translation I. Small non-coding RNAs.

11. Strategies and mechanisms in regulation of translation II. Translation attenuation. Translational frameshifts.

12. Gene regulatory networks. General properties of gene regulatory networks.

13. Model systems of regulatory networks. The lysis-lysogeny decision of bacteriophage lambda.

14. Models of gene regulatory networks. Approaches in modeling. Elementary network motifs.

• Anthony J.F. Griffiths, Susan R. Wessler, Sean B. Carroll, John Doebley „An introduction to genetic analysis”, 10th edition, W. H. Freeman, 2010

• SF Gilbert „Developmental Biology”, 7th edition, Palgrave Macmillan, 2003

• EH Davidson „Genomic regulatory systems”, Academic Press 2002

• B Lewin „Genes”, Jones & Bartlett Learning 2005

• Jocelyn E. Krebs, Benjamin Lewin, Elliott S. Goldstein, Stephen T. Kilpatrick: Lewin’s Essential Genes, Jones & Bartlett Publishers, 2013, ISBN 9781449612658