SUBJECT

Programmed Cell Death and Autophagocytosis

lecture

master

Faculty of Science

Biology MSc

4

Semester 2

Spring semester

1. Introduction: the role of cell death in physiological and pathological processes

2. Apoptosis

3. The discovery and molecular mechanism of apoptosis in C. elegans

4. The role of the caspase cascade in apoptosis

5. Cell death receptors, extracellular regulation of cell death

6. The discovery of the Bcl-2 protooncogen family, and their functions

7. The regulatory role of the mitochondria in the apoptosis, the intracellular regulatory mechanisms

8. The structure and functions of the ubiquitin protein

9. The structure and function of the proteasome and its subunits

10. The connections between the ubiquitin-proteasome system and cell death

11. The role of heath shock proteins in cell death

12. Apoptosis in the immune system

13. Autophagy

14. The lysosomes and the lysosomal compartment

15. Formation of the autophagosomes, fusion of autophagosomes and lysosomes, degradation in autophag vacuoles

16. Endocytosis and heterophagocytosis

17. Genetic regulation of the autophagy in yeast by Atg genes

18. The role of the TOR kinase in yeast and multicellular organisms

19. Autophagy and autophagy genes in C. elegans

20. Autophagy and autophagy genes in Drosophila

21. The role of the autophagy during the development of vertebrates

22. Autophagy and neurondegeneration

23. The role og autophagy in stroke, myopathies and cancer

24. Molecular mechanisms of heterophagocytosis

25. The role of the autophagy at the level of the organizm (death or survival, lifespan)

26. Methods and markers of apoptotic and autophagocytotic processes.

• Autophagy, Ed.: Klionsky DJ, Landes Bioscience, Georgetown, Eurekah. Austin, Texas, U.S.A.; 2004

• Molecular Biology of the Cell, Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K. and Walter, P. 4th Edition Garland Science New York, 2002