SUBJECT

Methods in Immunology I

practical

master

Faculty of Science

Biology MSc

4

Semester 2

Spring semester

1. Detection of serum proteins by immunoelectrophoresis.

2. Specific antibody titer detection by double diffusion in gel (Ouchterlony),

3. by agglutination and by enzyme immuno assay (ELISA).

4. Concentration determination of serum proteins by single radial immunodiffusion (Mancini) and by enzyme immuno assay (ELISA).

5. Total haemolytic complement assay.

6. Preparation of cell suspension from mouse spleen, bone marrow, thymus and lymph nodes. Detection of immature/mature and T- cell - B-cell ratio by flow cytometry (FACS).

7. Detection of in vitro proliferative capacity of polyclonally activated (LPS és ConA) mouse spleen cells by 3H-thymidine incorporation.

8. Phagocytosis of opsonized yeast by mouse macrophage cells.

9. Culturing of mouse hybridoma and myeloma cells in HAT selective medium. Comparison of aminoptrine sensitivity between the Sp2 myeloma and hybridoma cells.

• Practical Immunology. F.C.Hay and O.M.R.Westwood. 4th ed. Blackwell Science, 2002.