SUBJECT

Title

Genetic Analysis

Type of instruction

lecture

Level

master

Part of degree program
Credits

2

Recommended in

Semester 2

Typically offered in

Spring semester

Course description

1. From the cistron to the complex gene.

2. Pedigree for Genetics,Genomics,Molecular Biology,Bioinformatics

3. Generalization of the allele concept, beyond the phenotypic variability, DNA polimorphysms, paralogy,orthology

4. Bacterium genetics: the partial diploid, linking DNA technology to classical bacterium genetics

5. Bacteriophage genetics : thinking in phage and diploids. Complementation, recombination, crossing over, gene conversion, genetic mapping. Phage genetics and DNA technology.

6. Plasmids, Transposons, phage genes : combinations and utilizations in the cloning aera.

7. Balanced lethality: complementation, balancer chromosomes, thoroughbred heterozygotes

8. Dissecting strutures and genetic pathways : epistases, suppression, inter-allelic complementation, phage and yeast di-hybrid set ups.

9. Gene silencing and phenocopy

10. Hybrid disgenesis, D.melanogaster, C.elegans, transposons P, Tc1, gene tagging, dominant-negative technique.

11. Bacteriophage lambda : the lambda paradigm for genetic regulation ,genetic switch, system biology, cooperative interactions, recruiting, link to developmental genetics

12. Tetrad analysis: gene conversion, crossing over, repair, half tetrad analysis, models for recombination.

13. The focus of mutation: gynander analysis , somatic crossing over –genetic mosaicism, blastoderma fate mapping in D.melanogaster.

14. Transgenic organisms: in genetic analyses, in bioindusrtry, agriculture, medicine

15. Historical genetics: mt genetics, Y chromosome genetics, hapmap

Readings
  • Anthony J.F. Griffiths, Susan R. Wessler, Sean B. Carroll, John Doebley „An introduction to genetic analysis”, 10th edition, W. H. Freeman, 2010

  • Anthony J.F. Griffiths: Modern Genetic Analysis, W. H. Freeman, 2002

  • A.H. Sturtevant: A History of Genetics, CSHL Press, 2001

  • hand-out printed materials from the lecturer