SUBJECT

Experimental Methods in neurobiology II.

lecture

master

Faculty of Science

Biology MSc

2

Semesters 1-4

Autumn/Spring semester

1. Miscellenous imaging techniques: FMRI, MRI, CT PET

2. Taking samples from brain tissue: Microdissection Micro-punch Laser capture Basics of sample preparation

3. Methods of neurochemistry Binding-assay Cell-frakcioning, studies on liposomes, sinaptosomes Stop-flow, receptor kinetics Izotop technique Fluorescent tags Microdialysis Analythical HPLC: theories, equipment, columns, eluents Measuerement of bioactive compounds from the extracellular space (monoamines, amino acids, nucleosides, peptids) LC-MS, electrospray ionization (ESI), field of application

4. Systems biology techniques Gen chipping Data base search and in silico biology Gen hybridization on chip, chip-PCR Planning of experiments Separation: 2D-electrophoresis, differencial electrophoresis (DIGE) Sample praparation, digestion (manual, automatic) Protein identification: MS: equipment: MALDI, SELDI, Tandem MS, Q, Q-TOF, Q-TOF-TOF MS sequenation, protein identification Protein network modeling: CYTOSCAPE, interaction databases

• G.B. Smejkal, A. Lazarev Separation Methods in Proteomics, Taylor&Francis, NY, 2006

• S. Srivastava Informatics in Proteomics, Taylor&Francis, NY, 2005

• J.H. Gross Mass Spectrometry, Springer, 2004