SUBJECT
Environmental Microbiology Practice
practical
master
3
Semesters 1-4
Autumn/Spring semester
The goal of the practical is to get acquainted with microbiological analytical tools for the analysis of environmental changes due to pollutions, moreover the demonstration of basic environmental biotechnologies in laboratory model systems, and in operating plants.
1. Monitoring of hydrocarbon degrading communities in polluted soil/water systems. Analysis of germ count and activity investigation results.
2. Detection of pollutant decomposition through the analysis of microbial metabolism. Techniques of substrate induced respiration, investigation of specific enzyme activities.
3. Use of respirometry techniques for the estimation of the rapidity of degradative processes: BOD measurement, methanogenesis.
4. Construction of a simple sewage treatment model system, its microbiological and technological characterisation. Visit to the South Pest Sewage Treatment Plant, investigation of the technologies.
5. Construction of a model composting system, microcosm experiment for remediation. Monitoring of the microbiota. Visit to a composting plant / a remediation plant, investigation of the technologies.
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Christon J. Hurst et al.: Manual of Environmental Microbiology, American Society for Microbiology Press, 2007