SUBJECT
Physical Analysis of Proteins
practical
master
3
Semesters 1-4
Autumn/Spring semester
The laboratory practical is held during one week in blocks .
1. Circular dicroism spectroscopy.
Far-UV and near-UV CD spectra of proteins.
Indirect study of secondary and tertiary structure of proteins
Effect of ligand binding, temperature, pressure and denaturing agents on the spectra
2. Differential scanning calorimetry.
Study of thermodynamic parameters of protein's 3-dimensional structure.
Temperature-dependent unfolding experiments.
3. In silico studies of proteins.
Protein structure databases.
Molecular dynamic simulation.
Studies of site-directed mutations by modelling
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Martin Chalfie, Steven R. Kain: Methods of Biochemical Analysis, John Wiley & Sons, 2005
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Ronald Reid: Peptide and Protein Drug Analysis, CRC Press, 1999