SUBJECT

Mycology

lecture + practical

master

Faculty of Science

Biology MSc

2

Semesters 1-4

Autumn/Spring semester

1. Structure and characteristics of the chromosomal and extrachromosomal genomes of fungi - Saccharomyces cerevisiae as a model. Genetic and physical maps

2. Structure and function of chromosomes, molecular karyotyping

3. Structure and function of mitochondrial DNA, mitochondrial mutants

4. Cytoplasmic and mitochondrial plasmids of fungi

5. Fungal genes of viral origin. Killer phenomenon. Transpozons in fungi

6. Chromosomal and mitochondrial inheritance of yeasts

7. Sexual processes in fungi. Genetics of mating in Saccharomyces cerevisiae.

8. Sexual and vegetative sections of life cycles in different groups of fungi I. Zygomycota, Ascomycota

9. Sexual and vegetative sections of life cycles in different groups of fungi II. Basidiomycota

10. Parasexual cycles of fungi. Heterokaryosis in Aspergillus nidulans

11. Protoplast induction and fusion

12. Molecular cloning in fungi; Cloning vectors in fungi

13. Identification of recombinant clones and molecules. Application of microfungi for the production of heterologous proteins

14. Molecular analysis, diagnosis and identification of human pathogenic fungi

• Bainbridge, B.W. Genetics of Microbes. Springer Science & Business Media, 2013

• Bos, C.J. (ed) Fungal Genetics. Principles and Practice. Marcel Decker, New York, 1996

• Leong, S.A., Berka, R.M. Molecular industrial mycology. Systems and applications for filamentous fungi. Mycology series Vol. 8. Marcel Dekker, Inc. New York, 1991

• Kück, U. II: Genetics and Biotechnology. (in: The Mycota. Eds: Esser, K., Lemke, P.A.) Springer Science & Business Media, 2004