SUBJECT

Title

Hyphenated Techniques for Element Speciation

Type of instruction

lecture

Level

Master

Faculty

Faculty of Science

Part of degree program

Doctoral School of Environmental Sciences

Chemistry MSc

Credits

Recommended in

Semester 2/4

Typically offered in

Spring semester

Course description

General characterization of off-line (HPLC-GF-AAS, HPLC-TXRF, TLC/OPLC-TXRF, TLC-LA-ICP-MS) and on-line techniques (HPLC-FAAS, HPLC-ICP-AES, HPLC-ICP-MS, GC-AAS, LC-QF-AAS) for element speciation in environmental chemistry; speciation of As, Cd, Cr, Hg, Ni, Pb and Sn in environment. Detailed course outline: 1. Reasons for doing elemental speciation; 2. Sampling strategies for elemental speciation: sampling of environmental samples; 3. Sampling strategies for elemental speciation: sampling of clinical samples; 4. Sample preparation for elemental speciation: derivatization and pre-concentration methods;5. Hyphenation techniques for volatile samples (GC as separation technique); 6. Hyphenation techniques for liquid samples (HPLC and CE as separation techniques); 7. Speciation techniques for solid samples: X-ray diffraction and XANES; 8. Toxicity of inorganic and organic arsenic species;9. Arsenic speciation in plant compartments; 10. Organotin compounds: from their discovery to their use as antifouling paints; 11. Toxicity of organotin compounds; 12. Toxicity of inorganic and organic mercury compounds. Minamata disease; 13. Biomonitoring for mercury exposure; 14. Lead toxicity. Saturnism;15. Toxicokinetics of lead;16. Biomarkers of lead exposure; 17. Speciation of chromium.

Readings

Handbook of Elemental Speciation (Eds.: R. Kertulis, H. Crews, J. Caruso, K.G. Heumann), Wiley, 2005
Modern Methods for Trace Element Determination (Eds.: C. Vandecasteele and C.B. Block), Wiley, 1995
Practical HPLC method development (Eds.: L.R. Snyder, J.J. Kirkland, Glajch J.L.), Wiley, 1997
Atomic Absorption and Plasma Spectrometry (Ed.: J. R. Dean), Wiley, 1997